Table 1. The Design of Forward and Reverse Primers .

Name of Primer	Description	Sequence of Primer	GC Content (%)	Tm (°C)
hG-CSF1	Not modified	5’TATACATATGACCCCGCTTGGCCCTGCGAGC 3’	58.1	67.3
hG-CSF2	5’-ends terminally modified	5’TATACATATGACACCACTTGGCCCAGCAAGCTCCCTGC 3’	52.6	67.8
hG-CSF3	5’-ends terminally modified	5’TATACATATGACACCACTTGGCCCTGCATCATCTCTGCCCC 3’	51.2	67.8
R	Reverse primer	5’ATGACTCGAGTCATTACGGCTGCG CCAGATGACGC 3’	57.1	58.6