Table 3. Minimum free energy for mRNA secondary structure formation around the 5’-terminal end .

ORF	Nucleotide sequences	Minimum Free Energy (Kcal/mol)
		RNAfold	Kinefold
hG-CSF1	CC TCT AGA AAT AAT TTT GTT TAAC TTT TAG GAG GTA AAA CAT ATG ACC CCG CTT GGC CCT GCG AGC AGC CTG CCC	-12.4	-15.9
hG-CSF-2	CC TCT AGA AAT AAT TTT GTT TAAC TTT TAG GAG GTA AAA CAT ATG ACA CCA CTT GGC CCA GCA AGC TCC CTG CCC	-8.9	-12.4
hG-CSF-3	CC TCT AGA AAT AAT TTT GTT TAAC TTT TAG GAG GTA AAA CAT ATG ACA CCA CTT GGC CCT GCA TCA TCT CTG CCC	-7.3	-10.8

Note: The sequences in blue color are ribosome binding site.