Fig. 1.

Schematic diagram of HvHPT and HvHGGT genes targeted by four specific gRNAs. (A) Gene structure of HvHPT with gRNA1 and gRNA2 targeting sites. The distance between the two target sites is 831 bp. (B) Gene structure of HvHGGT with gRNA3 and gRNA4 targeting sites. The distance between the two target sites is 304 bp. (C) Schematic of the tRNA–gRNA fragments inserted into the pRGEB32 vector. Black rectangles indicate protein-coding regions. The PAM, protospacer-adjacent motif, is shown in red. The green vertical lines indicate the relative location of gRNA targeting sites; the overlined nucleotides indicate the target sequence for each gRNA. pUBI10, rice ubiquitin promoter; pU3, rice U3 snoRNA promoter.