Table 2.

Selection and characterization of ‘transgene-free’ homozygous barley mutants

T-DNA constructs	T1 mutant lines	No. of plants tested	No. of Cas9-free plants	No. of heterozygotes (Cas9-)	No. of homozygotes (Cas9-)	Mutation pattern of homozygotes (Cas9-)
PTG-HPT/Cas9	hpt11	146	27	4	2	+1 bp (+G); −1 bp (−A)
	hpt14	54	12	7	5	−9 bp (−CGAAACGGG)
	hpt15	48	21	13	7	−746 bp (large-fragment deletion)
PTG-HGGT/Cas9	hggt5	52	15	0	15	+1 bp (+A); +1 bp (+T); −1 bp (−G)
	hggt10	50	16	2	14	+1 bp (+A); −1 bp (−G); +1 bp (+A)/ −1 bp (−G)
	hggt13	44	15	0	15	+1 bp (+T)
	hggt15	54	17	4	7	+1 bp (+A)