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. Author manuscript; available in PMC: 2021 Feb 12.
Published in final edited form as: Cell Host Microbe. 2020 Feb 5;27(2):249–261.e5. doi: 10.1016/j.chom.2020.01.003

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Mouse Monoclonal Antibody-18A7 This study N/A
Mouse Monoclonal Antibody-14B10 This study N/A
Mouse Monoclonal Antibody-NA9D7 This study N/A
Bacterial and Virus Strains
CVA16/190 strain This study (Infectious clone) GenBank no. KJ850274
CVA16/213a strain This study (Infectious clone) GenBank no. JX127259
CVA16/4430 strain This study (Infectious clone) GenBank no. JX127274
CVA16/4479 strain This study (Infectious clone) GenBank no. JX127277
Chemicals, Peptides, and Recombinant Proteins
SYTO9 Invitrogen S34854
Critical Commercial Assays
QIAamp Mini viral RNA Extraction Kit Qiagen 52904
Deposited Data
Cryo-EM structure of CVA16 mature virion This paper EMD-0887, PDB: 6LHA
Cryo-EM structure of CVA16 A-particle This paper EMD-0888, PDB: 6LHB
Cryo-EM structure of CVA16 empty particle This paper EMD-0889, PDB: 6LHC
Cryo-EM structure of CVA16-M:18A7 This paper EMD-0890, PDB: 6LHK
Cryo-EM structure of CVA16-A:18A7 This paper EMD-0891, PDB: 6LHL
Cryo-EM structure of CVA16-E:18A7 This paper EMD-0892, PDB: 6LHO
Cryo-EM structure of CVA16-M:18A7-local This paper EMD-0897, PDB: 6LHT
Cryo-EM structure of CVA16-M:14B10 This paper EMD-0894, PDB: 6LHP
Cryo-EM structure of CVA16-M:NA9D7 This paper EMD-0895, PDB: 6LHQ
Cryo-EM structure of CVA16-M:18A7:14B10:NA9D7 This paper EMD-0898
Experimental Models: Cell Lines
Myeloma cells (Sp2/0-Ag-14) This study N/A
RD cells ATCC CCL-136
Experimental Models: Organisms/Strains
BALB/c mice Slac Laboratory Animal Co., shanghai, China N/A
Software and Algorithms
Prism 7.0 GraphPad Software N/A
MotionCor2 (Zheng et al., 2017) http://msg.ucsf.edu/em/software/motioncor2.html
Gctf (Zhang, 2016) https://en.wikibooks.org/w/index.php?title=Software_Tools_For_Molecular_Microscopy&stable=0#Gctf
RELION2.1 (Kimanius et al., 2016) http://www2.mrc-lmb.cam.ac.uk/relion
ResMap (Kucukelbir et al., 2014) http://resmap.sourceforge.net
AUTO3DEM (Yan et al., 2007) http://cryoem.ucsd.edu/wikis/software/start.php?id=auto3dem:home
Chimera (Pettersen et al., 2004) http://www.cgl.ucsf.edu/chimera
PyMOL (Schrodinger, 2010) https://pymol.org/2/
cryoSPARC (Punjani et al., 2017) https://cryosparc.com
ChimeraX (Goddard et al., 2018) https://www.cgl.ucsf.edu/chimerax/
COOT (Emsley and Cowtan, 2004) http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot
PHENIX (Adams et al., 2010) http://phenix-online.org
Molprobity (Chen et al., 2010) http://molprobity.biochem.duke.edu
CCP4 (Collaborative, 1994) http://www.ccp4.ac.uk
cisTEM (Grant et al., 2018) https://cistem.org
Scipion (De la Rosa-Trevín et al., 2016) https://scipion-em.github.io/docs/
Localized reconstruction (Ilca et al., 2015) https://github.com/OPIC-Oxford/localrec
Rivem (Xiao and Rossmann, 2007) http://bilbo.bio.purdue.edu/~viruswww/Rossmann_home/softwares/river_programs/rivem.php
Other
Quantifoil R 2/2 Cu grids Quantifoil Q2100CR2