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. 2020 Oct 7;11:20. doi: 10.1186/s13227-020-00166-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Experimental techniques commonly used in anemonefish. a–c Multicolor FISH in the retina, showing that RH2A (green) and RH2B (magenta) are expressed in opposite members of every double cone throughout the retina. Scale bar 10 µm.[72]. d Pictures of an embryo after successful injection of red dye. e Scanning electron microscopy of the larval head of Amphiprion ocellaris. f–k Pictures of wild-type (f) and color pattern mutants of Amphiprion ocellaris (g–k)