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. 2020 Oct 7;51:127. doi: 10.1186/s13567-020-00854-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Adherence of WT C83902 and C83902 ΔznuACB. A Deletion of C83902 znuACB decreases the adherence to IPEC-J2 cell. An adherence assay of WT C83902 and C83902 ΔznuACB to IPEC-J2 cell after pre-incubation in LB medium. B Zinc restriction led to a significant decrease of adhesion ability of C83902 ΔznuACB, while zinc supplementation can restore its adhesion ability. Bacteria were incubated in 30 μM TPEN pre-treated LB medium and supplemented with 10 μM or 20 μM Zn²⁺, then adherence assays of WT C83902 and C83902 ΔznuACB to IPEC-J2 cell were performed. The WT strain adhesion index was assumed to be 100%. Data are expressed as mean ± standard deviation of triplicate experiments. Statistically significant differences are indicated as ***p < 0.001 when compared to the WT strain.