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. 2020 Sep 23;11:562795. doi: 10.3389/fimmu.2020.562795

FIGURE 3.

FIGURE 3

Accumulation of IgT+ B-cells in the BM of trout infected with F. columnare. (A) Representative DIC images of immunofluorescence staining on paraffinic sections of BM from uninfected control fish (left), infected fish (28 dpi, middle), and immune fish (75 dpi, right). IgT+ and IgM+ B-cells were stained with rabbit anti-trout IgT (green) and mouse anti-trout IgM (red), respectively; nuclei were stained with DAPI (blue; isotype-matched control antibody staining, Supplementary Figure S4 in Supporting Information). (B) The number of IgT+ and IgM+ B-cells in paraffinic sections of BM from uninfected control fish, infected fish, and immune fish (n = 6 per group), counted in 20 fields from (A) (original magnification, × 20). (C,D) Concentration of IgT, IgM, and IgD in buccal mucus (C) and serum (D) of control, infected, and immune fish (n = 12 per group). BC, buccal cavity; BE, buccal epithelium; and LP, lamina propria. Scale bar, 20 μm. *P < 0.05, **P < 0.01, and ***P < 0.001 (one-way ANOVA with Bonferroni correction). Data in (B–D) are representative of at least three independent experiments (mean ± SEM).