FIGURE 3.

Binding of mG2-scFv to endogenous ICAM1 in breast cancer/normal cell lines. (A) Expression of ICAM1 in human breast cancer cells and normal breast cell were evaluated by FACS. Cells were incubated with anti-ICAM1-FITC (red) or IgG-FITC control (green). (B) Quantification of ICAM1 staining intensities in various breast cancer cells and normal breast cells. Unpaired two-tailed Student’s t-tests were applied. *p < 0.05, ***p < 0.001. (C) IF staining with mG2-scFv-Fc in target cells. Scale bars, 50 μm.