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. 2020 Sep 1;10(9):2832–2850.

Figure 2.

Figure 2

SLFN5 inhibits BRCA cell proliferation and promotes apoptosis. A. Proliferation analysis of BRCA cells with altered SLFN5 expression was performed by Ki67 immunofluorescence detection. B. Representative images of colony formation in BRCA cells with altered SLFN5 expression. C. Apoptosis analysis of MCF-7 cells with silenced SLFN5 expression was performed by flow cytometry. D. Apoptosis analysis of MDA-MB-231 cells overexpressing SLFN5 was performed by flow cytometry. E. Apoptosis analysis of MCF-7 cells with silenced SLFN5 expression was performed by a TUNEL assay. F. Apoptosis analysis of MDA-MB-231 cells overexpressing SLFN5 was performed by a TUNEL assay. G. The expression of caspase-3, cleaved caspase-3 and Bax in BRCA cells with altered SLFN5 expression was determined using Western blotting. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar = 100 μm.