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. 2020 Sep 1;10(9):2878–2894.

Figure 1.

Figure 1

PD-L1 is an unpredictable biomarker of response to PD-1/PD-L1 Inhibition. (A) PD-L1 mRNA and protein expression levels and (B and C) surface PD-L1 expression in MCF7, BT20, and MDA-MB-231 cells; (D) western blot analysis for PD-1 expression and ELISA assay for IL-2 and IFNγ levels in activated Jurkat T cells after PMA (50 ng/mL) and Ionomycin (1 µg/mL) treatment; (E) Caspase 3/7 activity of PD-L1(-) MCF7 cells, co-cultured with activated Jurkat T cells; Caspase 3/7 activity of PD-L1(+) (F) BT20 and (G) MDA-MB-231 cells, co-cultured with activated Jurkat T cells, after treatment of PD-1/PD-L1 inhibitor for 24 h.