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. 2020 Sep 1;10(9):2878–2894.

Figure 2.

Figure 2

TG2 induces PD-L1 expression by blocking PTEN and activating NF-κB in TNBC cells. (A) Fold change in TG2 in MDA-MB-231 and BT20 cells, and western blot analysis of TG2, PD-L1, PTEN, pAKT, and IκBα in MCF7 and MDA-MB-231 cells, with β-Actin as a loading control. (B) Western blot analysis of TG2, PD-L1, PTEN, pAKT, and IκBα in MCF7/Mock, MCF7/TG2, MDA-MB-231, and MDA-MB-231/TG2 siRNA cells, with β-Actin as a loading control. (C) Cell Immunohistochemistry and (D) FACS analysis for surface PD-L1 expression level in MCF7/Mock, MCF7/TG2, MDA-MB-231 and MDA-MB-231/TG2 siRNA. (E) Incidence of TG2 and PD-L1 expression in Formalin-Fixed Paraffin-Embedded (FFPE) tissue samples of 648 TNBC patients.