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. 2020 Sep 1;10(9):2785–2799.

Figure 6.

Figure 6

Overexpression of ASK1 inhibits nuclear localization of TAZ, but not YAP. Lung cancer A549 cells stably expressed ASK1 or its kinase-dead mutant were cultured on glass cover-slips, serum-starved 24 hrs followed by stimulation with 10% serum for 30 min. A. The cells were fixed and immuno-stained with the anti-YAP or the anti-TAZ antibody, and the nuclei were stained with fluorescent dye DAPI. B. The expression of ASK1 and its kinase-dead mutant was detected by immunoblotting of the cell lysates with an anti-ASK1 antibody. Bar, 20 mm.