FIGURE 1.

SP1 is upregulated in RGCs isolated from ONC‐injured rat retina. A, Heat map of expression of TFs in RGCs from sham and ONC rats after 4 d. B, Functional serial deletion analyses of the LINGO‐1 promoter. LINGO‐1 promoter 5′ sequential deletion constructs were created and fragments of the LINGO‐1 promoter of different lengths were cloned into the pGL3‐Basic plasmid. HEK293 cells were cotransfected with the LINGO‐1 promoter constructs and pCMV‐luc, incubated for 24 h, and luciferase activity was assayed and normalized to pGL3‐Basic (n = 3, *P < .05, and compared to pGL3‐basic control by ANOVA with the LSD multiple comparison test. C, Two putative SP1 binding sites at nucleotides −649 to −641 and −508 to −500 of the LINGO‐1 promoter. D, Relative mRNA level of SP1 in RGCs at 0, 4, and 7 d post‐ONC by qRT‐PCR (n = 4, means ± SD, compared to 0 d by ANOVA with the Tukey multiple comparison, *P < .05)