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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: FASEB J. 2019 Nov 29;34(1):1247–1269. doi: 10.1096/fj.201902163R

Figure 8: Effect of ppp3r2 deletion on sperm ATP levels and distribution of monocarboxylate transporter protein complex.

Figure 8:

A) Wild type and knock out sperm were incubated in presence or absence of the indicated energy substrates for 1 hour and ATP levels were determined by a luciferase assay as described. ATP level in the ppp3r2 knockout spermatozoa incubated in presence of pyruvate did not increase as opposed to that of WT sperm population. B) Expression of MCT2 and its binding partner, basigin in calcineurin KO mouse sperm were significantly lower as seen in western blot of whole sperm lysate (upper panel) and immune-cytofluorescence (lower panel) developed by anti-mouse MCT2 and anti-goat EMMPRIN (basigin) antibodies. As loading control for the western blots, mouse monoclonal anti-β-actin antibodies were used; the calculated average band intensities (n=3) provided on top of the corresponding blots. For immunofluorescence, cyanine 3 and Alexa fluor488 conjugated secondary IgGs were used for MCT2 and basigin, respectively; Hoechst was used for DNA staining. Details of the protocol has been described in materials and methods. Merged picture is in the right-hand corner.