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. 2020 Jul 9;59(37):16225–16232. doi: 10.1002/anie.202002147

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© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Glycation graph assembled from glycosylation and glycation data on bevacizumab. Each node (gray) represents a proteoform with a unique monosaccharide composition and is labeled by a glycoform that is compatible with this composition. Arrows link nodes whose monosaccharide compositions differ solely in their numbers of hexoses. Arrows emerging from nodes 6 and 7 (upper right) are labeled by those differences; all other arrows share this color scheme. (Node numbers in this connected component correspond to the number of hexoses in the respective proteoforms.) The version of Equation (1) (see Supporting Information) that applies to node 8 (pink) describes all the parameters that influence its observed abundance.