Figure 2.

Comparison of automated versus manual washing of the antigen‐antibody‐bead complexes. A) Comparison of the efficiency of manual versus automated bead washing for enriching PTEN and p110α dSIS peptide (2.5 fmol) spiked into E. coli digest. The signal of the stable isotope labelled AKT2 peptide (THF₍₊₁₀₎PQFSYSASIR₍₊₁₀₎E) spiked into the MALDI matrix (1 fmol per spot) was used for normalization. Error bars represent standard deviation, horizontal bars indicate means. Values above and below the data points represent the mean and absolute standard deviation; N = 5. B,C) Mass spectra of antigen‐antibody‐bead complex using B) manual and C) automated bead washes, with data recorded in the reflectron mode, showing similar non‐specific backgrounds in both bead‐washing methods.