Fig. 1.

Vacuolar iron Transporter‐Like (VTL) proteins in Medicago truncatula. (a) Phylogenetic relationship and predicted domain structure of VTL protein sequences identified in the M. truncatula genome and those previously reported in Arabidopsis thaliana (AtVTL1, AT1G21140; AtVTL2, AT1G76800; AtVTL3, AT3G43630; AtVTL4, At3G43660; AtVTL5, AT3G25190). The ‘true’ Vacuolar Iron Transporter (VIT) sequences including Ccc1 from Saccharomyces cerevisiae served as an outgroup. The domain structure of VIT proteins is adapted from Kato et al. (2019). (b, c) Expression of M. truncatula VTL genes in roots and nodules (b) and in different nodule zones (c). Data were obtained from the Symbimics website (https://iant.toulouse.inra.fr/symbimics), and are Ribominus‐like values for (b) and DESeq‐normalized reads from laser‐capture microdissection fractions for (c). ZIId and ZIIp, distal and proximal fractions of Zone II, respectively. IZ, Interzone. ZIII, nitrogen‐fixing Zone III. Error bars represent SE.