Fig. 4.

VTL8 is required for development of functional nodules. (a) Wild‐type Medicago truncatula plants (WT, Jemalong) and the 13U mutant transformed with VTL4, VTL8, VTL4 + VTL8 or empty vector (e.v.), as indicated. Roots were inoculated with Sinorhizobium medicae and images taken 31 d post inoculation (dpi). Bar, 1 cm. (b) Root nodules from plants in (a). Transformed roots were confirmed by DsRed fluorescent protein marker (not shown). Bars, 1 mm. (c) Wild‐type; (d), 13U + e.v. and (e), 13U + VTL8, longitudinal sections of nodules stained with SYTO13. Bars, (c–e), 200 μm.