Figure 3.

CD9 regulates cell surface trafficking and α-secretase activity of ADAM family in PDAC. A. Representative images and quantification of western blot showing CD9 protein levels after its lenti-viral knockdown in two PDAC cell lines. B. In-vitro FRET assay showing α-secretase activity was inhibited by CD9 knockdown in two cell lines. C. Cell surface biotin labeling results showing the protein levels of ADAM10, ADAM9 and ADAM17 on the cell surface were reduced by CD9 knockdown. D. Immunofluorescence images of FLAG antibody staining in non-permeabilized cells transfected with FLAG-ADAM10, FLAG-ADAM9 or FLAG-ADAM17 together with pcDNA3.1-CD9 or control vector. For all, **P<0.01, ***P<0.001.