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. 2020 Sep 15;12(9):5205–5220.

Figure 7.

Figure 7

OMST counteracted BLM-induced cellular senescence via miR-665 targeting of SDC1. BLM-induced VSMCs were treated with OMST plusmiR-665 mimics + SDC1 transfection. A. SDC1 protein expression was detected by western blotting. B. SDC1 mRNA expression was determined by quantitative real time PCR. C. The NAD+/NADH ratio was determined by a NAD+/NADH assay. D, E. Cell senescence was evaluated by staining for SA-β-gal-positive cells. Representative photomicrographs showing SA-β-gal-positive cells (blue) among VSMCs. F. Representative immunofluorescence images of nuclear γH2AX (cell nuclei: blue; γH2AX: red) in VSMCs from the control, BLM, BLM+OMST, BLM+OMST+miR-665 mimic, and BLM+OMST+miR-665 mimic+SDC1 groups. G-I. Cell proliferation and cell apoptopsis was assessed by the Edu flow cytometry assay and Annexin V/PI staining flow cytometry assay. ***P < 0.001 vs. control; #P < 0.05, ##P < 0.01 vs. BLM group; &P < 0.05 vs. BLM+OMST group; @P < 0.05 vs. BLM+OMST+miR-665 mimic group.