Figure 4.

CerS6 knockdown inhibits invasion and migration and promotes the apoptosis of serous ovarian cancer cells. (A and B) The level of invasion was probed in the CerS6 knockdown and negative control (NC) groups derived from (A) OVCAR3 and (B) OVCAR8 cells. (C) Quantification of invasion rates in OVCAR3 and OVCAR8 cells. (D and E) The level of migration was probed in the CerS6 knockdown and NC groups derived from (D) OVCAR3 and (E) OVCAR8 cells. (F) Quantification of migration rates in OVCAR3 and OVCAR8 cells. (G and H) Representative wound healing images of CerS6 knockdown (G) OVCAR3 and (H) OVCAR8 cells at 0 h and 24 h post-wounding. The cell boundary is indicated by the black outline. (I) Quantification of wound healing rates in OVCAR3 and OVCAR8 cells. Data are means ± SD of the wound area relative to the NC group. (J and K) CerS6 knockdown promotes (J) OVCAR3 and (K) OVCAR8 cell apoptosis, as detected by cell apoptosis assays. (L) Quantification of apoptosis rates in OVCAR3 and OVCAR8 cells. Scale bar: 100 μm. (The values represent mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001. Experiments were statistically analyzed using two-tailed Student’s t-test).