Figure 2.

FOXA2 knockdown inhibits PC3 cell migration but not proliferation. A. RT-qPCR analyses were conducted to assess the expression levels of FOXA2 in PC3/Control and PC3/FOXA2-KD cells. The PC3/FOXA2-KD cells demonstrated reduced mRNA levels compared to the PC3/Control cells. B. Western blot analysis confirmed decreased FOXA2 expression in PC3/FOXA2-KD cells. β-actin served as the loading control. C. Cell proliferation assays were conducted using the IncuCyte zoom method. Pooled FOXA2 knockdown cells were used in this experiment. FOXA2 knockdown did not decrease the proliferation of PC3 cells. D. Chemotaxis assays. PC3/Control and PC3/FOXA2-KD cells were seeded on collagen I-coated transwell inserts with 0.5% FBS-containing media in the top chamber and 10% FBS-containing media in the bottom wells. The bottoms of the collagen-coated inserts were imaged every two hours to visualize the cells that had traversed. PC3/Control cells gradually migrated through the collagen I matrix into the bottom wells, whereas PC3/FOXA2-KD cells could not do so. Error bars reported as standard deviation.