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. 2020 Sep 15;12(9):5619–5629.

Figure 6.

Figure 6

FOXA2 regulates the expression of PTHLH. A. RT-qPCR to assess the expression of PTHLH. The mRNA levels of PTHLH is lower in PC3/FOXA2 knockdown cells. *P<0.01, t-test. Error bars reported as standard deviation. B. RT-qPCR to assess the expression of PTHLH. FOXA2 over expression in mouse prostate prostatic epithelial cells caused an increase in the mRNA levels of PTHLH. *P<0.05, t-test. Error bars reported as standard deviation. C-E. Chromatin Immunoprecipitation analysis. ChIP assays were conducted by using PC3/Control and PC3/FOXA2-KD cells. FOXA2 bound to both FOXA2 binding sites (A2BS1 & A2BS2) in the regulatory region of the PTHLH gene. Additionally, H3K4me3 (histone mark of active promoters) and H3K27Ac3 (histone mark of active enhancers) were enriched in the PTHLH regulatory regions. (*P<0.01, t-test).