Figure 5.
Effects of various treatments on the survival and apoptosis of CRC cells. A. The survival rate was detected by MTT assay in HT29 and HCT116 cells after various radiation doses (0, 2, 4, 6, and 8 Gy) for 24 h. The LD50 of HT29 and HCT116 cells was approximately 6.2 Gy and 4.2 Gy, respectively. B. The survival rate was detected using MTT assay in HT29 and HCT116 cells transfected with siRNA-NC or siRNA-PFK1 after treatment with or without radiation (2 Gy or 6 Gy) for 24 h. C. The apoptosis rate was detected by flow cytometry in siRNA-PFK1-transfected HT29 and HCT116 cells after exposure to irradiation. Data are presented as mean ± SD (n = 3 independent experiments). PFK1, phosphofructokinase-1; siRNA-NC, negative control siRNA cells; siRNA-PFK1, PFK1 knockdown cells; IR, ionizing radiation. *P < 0.05, **P < 0.01, and ***P < 0.001.