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. 2020 Sep 15;12(9):4923–4940.

Figure 9.

Figure 9

Oligomycin A decreases the inhibitory effects of PFK1 knockdown on the survival and colony formation ability of HT29 cells with and without irradiation. A. In the absence or presence of irradiation, the cell survival rate was detected by MTT assay in siRNA-NC and PFK1 knockdown HT29 cells treated with or without oligomycin A. B and C. The colony survival fraction was detected using colony formation assay in HT29 cells treated with siRNA-NC, siRNA-PFK1, or siRNA-PFK1 plus oligomycin A after irradiation with 0, 2, 4, 6, or 8 Gy. Data are presented as mean ± SD (n = 3 independent experiments). PFK1, phosphofructokinase-1; siRNA-NC, negative control siRNA cells; siRNA-PFK1, PFK1 knockdown cells; oligo, oligomycin A. *P < 0.05, **P < 0.01, and ***P < 0.001.