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. 2020 Sep 15;12(9):5064–5079.

Figure 4.

Figure 4

FOXM1 docked onto the promoter of PUMA at the BS1 site to regulate its expression. A. The consensus sequence of FOXM1. B. The promoter of PUMA contained two putative FOXM1 binding sites. A 1500-bp length of the PUMA promoter was assessed for FOXM1 binding sites. Two binding sites (BS1 and BS2) were identified; their positions were shown. C. The relative luciferase activities. Three plasmids, pGL4.3-pPUMAWT, pGL4.3-pPUMA▽BS1 and pGL4.3-pPUMA▽BS2, were cotransfected with Renilla into Control-KD, FOXM1-KD1, FOXM1-KD2, Control-OE and FOXM11-OE cells. The transfected cells were cultured for an additional 24 h and then subjected to a dual-luciferase reporter assay. The relative luciferase activities were determined by normalizing the firefly luciferase activities to their corresponding Renilla activities. **P<0.01 and ***P<0.001. D. The occupancy of FOXM1 on the promoter of PUMA. The ChIP assays were performed in Control-KD, FOXM1-KD1, FOXM1-KD2, Control-OE and FOXM1-OE cells using anti-FOXM1 and IgG (negative control). The purified input and output DNA samples were subjected to RT-qPCR analysis to examine the occupancy of FOXM1 on the promoter of PUMA. **P<0.01 and ***P<0.001.