Figure 5.

Knockdown of FOXM1 failed to active apoptosis signaling following PDA treatment. (A) Western blotting results. The Control-KD (Control), FOXM1-KD1 and FOXM1-KD2 cells were treated with or without 0.3 mM PDA for 12 h, followed by total protein extraction. Western blotting analyses were performed to detect the protein levels of FAS, TRADD, p53, BIM, FOXM1, PUMA, BAX, BCL2, CASP3, CASP7, CASP9, and GAPDH (loading control). (B and C) The normalized protein levels. The protein signals as shown in (A) were quantified using ImageJ software and then normalized to their corresponding GAPDH levels. **P<0.05, **P<0.01 and ***P<0.001.