Figure 3.

Hypoxia pretreatment improves cell morphology, promotes cell proliferation, and colony formation, but does not affect apoptosis. A. Morphological comparison of USCs under normoxic and hypoxic culture conditions. B. Statistical analysis histograms and comparison of cell surface marker expression levels in USCs. C. Cell growth curve, colony formation assay, and its statistical analysis. D. Cell cycle and apoptosis measured by flow cytometry. *P<0.05, **P<0.01, ***P<0.001, Student’s t-test. Scale bar =100 μm.