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. 2020 Sep 15;12(9):5131–5150.

Figure 4.

Figure 4

Hypoxia pretreatment promotes cell migration but does not affect cell differentiation. The migration of USCs was determined by (A) wound-healing assay and (B) transwell migration assay, and its statistical analysis. (C) The differentiation potential of USCs was determined by alkaline phosphatase (ALP) staining, alizarin red staining, oil red O staining and Alcian blue staining. ***P<0.001, Student’s t-test. Scale bar =100 μm.