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3-Methyladenine (3-MA) and Bafilomycin (Baf) could reverse the effect of hypoxia-induced USC proliferation and migration by inhibiting autophagy. A. Autophagy-related markers were detected by western blot. B. Cell growth was measured by trypan blue staining. C. Cell proliferation was determined by colony formation assay. D. The migration of USCs was determined by a wound-healing assay and transwell migration assay. *P<0.05, **P<0.01, ***P<0.001, one-way ANOVA. Scale bar =100 μm.
