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. 2020 Sep 15;12(9):5696–5707.

Figure 4.

Figure 4

ADAMTS9-AS1 increased the PI3K/AKT/mTOR signaling pathway proteins in HCC cells in vitro. A, C. In HepG2 and MHCC97-H cells transfected with pcDNA3.1-AS1 groups, it suggested that overexpression of ADAMTS9-AS1 increased the expression levels of p-AKT, PIK3CB, and p-mTOR compared to their corresponding pcDNA3.1 vector groups. B, D. In HepG2 and MHCC97-H cells transfected with si1-AS1 or si2-AS1 groups, knockdown of ADAMTS9-AS1 decreased p-AKT, PIK3CB, and p-mTOR expression levels compared to corresponding their pcDNA3.1 vector groups. ADAMTS9-AS1, ADAMTS9 antisense RNA 1; p-AKT, phosphorylated AKT; p-mTOR, phosphorylated mTOR; NC, negative control; si, short interfering RNA.