Figure 5.

ADAMTS9-AS1 decreased autophagy and apoptosis in HCC cells in vitro. A, C. Western blot assays was conducted to examine the protein levels of autophagy and apoptosis-related proteins in HCC cells. Compared with corresponding control groups, the expression of LC3-II, BECN1, and pro-apoptotic Bax decreased in pcDNA ADAMTS9-AS1 groups compared to corresponding control groups, and no significant change was found in LC3-I expression. Besides, decrease of ADAMTS9 was also found in pcDNA ADAMTS9-AS1 groups. B, D. Increase of ADAMTS9, LC3-II, BECN1, and pro-apoptotic Bax was identified in si1-AS1 or si2-AS1 groups as compared with corresponding control groups. ADAMTS9-AS1, ADAMTS9 antisense RNA 1; NC, negative control; si, short interfering RNA; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.