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. 2020 Sep 15;12(9):5670–5682.

Figure 5.

Figure 5

The USP2-AS1 regulates Hippo-YAP1 signaling in COAD cells. (A-C) Luciferase reporter assays showed that USP2-AS1 silencing significantly inhibited TEAD transcriptional activity in LoVo (A) and SW620 (B), while USP2-AS1 overexpression enhanced TEAD transcriptional activity (C). (D) Western blot analysis showed that USP2-AS1 regulated the level of p-LAST1 (T1079), LAST1, LAST2, p-YAP1 (S127), and the total YAP1 in indicated cell lines. (E, F) The silencing of USP2-AS1 inhibited the expression of indicated Hippo signaling targets in SW620 cells (E), while its overexpression elevated the levels of these genes (F). (G-I) The expression of USP2-AS1 was positively correlated with CTGF (G), CYR61 (H) and SOX9 (I) in fresh COAD tissues. (J) Constitutively activated YAP1 (S127A) partly improved the proliferation of inhibitory effects through USP2-AS1 knockdown. Each bar represents the mean ± SD for n = 3.