Figure 3.
MiRNA-543 directly targeted on KLF6. (A) Binding sites in promoter regions of miRNA-543 and KLF6. (B) Luciferase activity in AGS and MKN45 cells co-transfected with NC/miRNA-543 mimics and KLF6-WT/KLF6-MUT. (C, D) The mRNA (C) and protein (D) levels of KLF6 in AGS and MKN45 cells transfected with NC or miRNA-543 mimics. (E) KLF6 level in gastric carcinoma tissues and adjacent normal tissues. (F) A negative correlation between expression levels of miRNA-543 and KLF6 in gastric carcinoma tissues.