Figure 1.

CD47 antibody inhibited ISO-induced cardiac hypertrophy. A. Representative H&E staining of mouse hearts from the four groups. B. Heart weight to body weight ratio (HW/BW) was significantly increased after ISO treatment, n=7 per group. C. The levels of fibrosis in the four groups were detected by Masson’s trichrome staining. D. Quantification of the volume of interstitial fibrosis was performed using Image-Pro Plus (n=6). CD47 antibody abolished the effects of ISO in increasing cardiac fibrosis. E, F. Atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) were increased in the ISO-induced mice which were reversed by CD47 antibody treatment. G, H. CD47 antibody treatment decreased CK-MB and LDH levels induced by ISO, n=7 per group. Data were analyzed by one-way ANOVA followed by Bonferroni’s post-hoc test and expressed as the mean ± SD. Statistical significance: *P < 0.05 and **P < 0.01 versus the IgG group; #P < 0.05 and ##P < 0.01 versus the IgG+ISO group.