Figure 3.

CD47 antibody protected against ISO-induced oxidative stress in cardiac tissues. A, B. CD47 antibody treatment increased the SOD activity and decreased the MDA level in serum induced by ISO, n=7 per group. C. The ROS levels in the hearts of mice from all groups were revealed by DHE staining of frozen sections. D. CD47 antibody treatment decreased ROS fluorescence intensity. The fluorescence intensity of DHE staining was analyzed using Image-Pro Plus, n=7 per group. Data were analyzed by one-way ANOVA followed by Bonferroni’s post-hoc test and expressed as the mean ± SD. Statistical significance: *P < 0.05 and **P < 0.01 versus the IgG group; #P < 0.05 and ##P < 0.01 versus the IgG+ISO group.