Figure 5.

CD47 antibody reduced the accumulation of autophagosome in ISO-treated H9c2 cells. A. Autophagic vesicles (AV) were detected by TEM in H9c2 cells subjected to ISO treatment. AVs by TEM were measured. The figures were representative images of three different experiments. B. CD47 treatment decreased AV number. AVs in different groups were quantified and presented as bar graphs, n=7 per group. Data were analyzed by one-way ANOVA followed by Bonferroni’s post-hoc test and shown as the mean ± SD. Statistical significance: *P < 0.05 and **P < 0.01 versus the IgG group, #P < 0.05 and ##P < 0.01 versus the IgG+ISO group.