CD47 antibody protected against ISO-induced injury through rescuing impaired autophagy flux. A. Representative Western blots photographs depicting protein levels of LC3 II, LAMP2, ATG5, ATG7, Cleaved caspase-3 and Cleaved caspase 9 in cardiac tissues. The figures were representative images of three different experiments. B. CD47 antibody promoted autophagy flow and inhibited apoptosis. Data were analyzed by one-way ANOVA followed by Bonferroni’s post-hoc test and shown as the mean ± SD, n=7 per group. Statistical significance: *P < 0.05 and **P < 0.01 versus the IgG group, #P < 0.05 and ##P < 0.01 versus the IgG+ISO group, respectively.