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. 2020 Sep 15;12(9):5908–5923.

Figure 7.

Figure 7

Blockage of autophagy reversed the protective effect of CD47 antibody on ISO induced H9C2 cells injury. A. Representative Western blots photographs depicting protein levels of LC3 II, LAMP2 and Cleaved caspase 9 in H9c2 cells. The figures were representative images of three different experiments. B. Autophagy inhibitor chloroquine (CQ) treatment reversed the autophagy flow promotion of CD47 antibody in H9c2 cells. The protein expression levels were quantitatively analyzed, n=7 per group. C. Representative images showing the LC3 fluorescent signals in H9C2 cells transfected with mRFP-GFP-LC3. D. LC3 dots in different groups were quantified and presented as bar graphs, n=7 per group. Data were analyzed by one-way ANOVA followed by Bonferroni’s post-hoc test and shown as the mean ± SD. Statistical significance: *P < 0.05 and **P < 0.01 versus the IgG group, #P < 0.05 and ##P < 0.01 versus the IgG+ISO group, &P < 0.05 and &&P < 0.01 versus the CD47+ISO group, respectively.