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. 2020 Sep 15;12(9):5955–5966.

Figure 3.

Figure 3

Functional role of CD44 expression in chemoresistance in colon cancer cells. A. Downregulation of CD44 expression inhibits cell growth under 5-FU treatment (500 μM) in SW480 cells (with CD44s protein). B. Downregulation of CD44 expression inhibits cell growth under 5-FU treatment (500 μM) in HT29 cells (with CD44v protein). C. Downregulation of CD44 expression enhances cleaved-PARP and cleaved-caspase 3 expression in SW480 cells under 5-FU treatment (500 μM) by western blot analysis at 48 h. β-actin is used as an internal control. D. Downregulation of CD44 expression enhances cleaved-PARP and cleaved-caspase 3 expression in HT29 cells under 5-FU treatment (500 μM) by western blot analysis at 48 h. β-actin is used as an internal control. E. Downregulation of CD44 expression inhibits cell growth under L-OHP treatment (20 μM) in SW480 cells (with CD44s protein). F. Downregulation of CD44 expression inhibits cell growth under L-OHP treatment (20 μM) in HT29 cells (with CD44v protein). G. Downregulation of CD44 expression does not affect protein expression levels of cleaved-PARP, cleaved-caspase 3, DPD, or ERCC1 in SW480 cells under L-OHP treatment (20 μM) by western blot analysis at 48 h. H. Downregulation of CD44 expression does not affect protein expression levels of cleaved-PARP, cleaved-caspase 3, DPD, or ERCC1 in HT29 cells under L-OHP treatment (20 μM) by western blot analysis at 48 h. MIA Paca-2 cells were used as a positive control for DPD expression.