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. 2020 Sep 15;12(9):5308–5319.

Figure 3.

Figure 3

Overexpression of miR-613 suppressed IL-1β-induced inflammatory response in CHON-001 cells. CHON-001 cells were transfected with miR-613 agonist for 48 h, and then exposed to 10 ng/mL IL-1β for 24 h. A-C. Expression levels of TLR4, p-p65 in CHON-001 cells were detected with western blot assay. Relative expression levels were quantified by normalization to β-actin and p65 respectively. D-F. ELISA assay was applied to measure the production of TNF-α, IL-6, and IL-8 in the supernatants of CHON-001 cells. **P < 0.01 compared with the control group; ##P < 0.01 compared with the IL-1β group.