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. 2020 Sep 15;12(9):5449–5464.

Figure 4.

Figure 4

TUG1 negatively regulated the expression of miR-26a in degenerated intervertebral disc nucleus cells. A. The expression of miR-26a was assessed by qRT-PCR in NPCs of control group and IDD group. B. The expression of miR-26a was examined in human degenerative disc NPCs transfected with pcDNA-TUG1 or si-TUG1. C. Sequence alignment of miR-26a and the putative binding sites within the wild-type TUG1, and mutation in the TUG1. D. The luciferase activity was detected in HEK293T cells transfected with TUG1-WT or TUG1-MUT reporter vector together with miR-26a mimics or miR-NC. E. The expression of miR-26a was examined in human degenerated intervertebral disc NPCs transfected with miR-NC, miR-26a mimic, pcDNA-NC+miR-26a or pcDNA-TUG1+miR-26a mimic. F. Proliferation of treated human degenerated intervertebral disc NPCs was detected by EdU assay. Scale bar =100 μm. G. Apoptosis of treated human degenerated intervertebral disc NPCs was detected by flow cytometry. H. The expression of collagenII, aggrecan, MMP-3 and MMP-13 were determined in treated human degenerated intervertebral disc NPCs by western blot assay. The original images are available in Figure S5. *P<0.05, **P<0.01.