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. 2020 Sep 15;12(9):5449–5464.

Figure 5.

Figure 5

TUG1 regulates miR-26a/HMGB1 axis in human degenerated intervertebral disc NPCs. (A) Sequence alignment of miR-26a and the putative binding sites within HMGB1-WT and HMGB1-MUT. (B) The luciferase activity was detected in HEK293T cells transfected with HMGB1-WT or HMGB1-MUT reporter vector together with miR-26a mimics or miR-NC. The expression of HMGB1 mRNA (C) and protein (D) was examined in human degenerated intervertebral disc NPCs transfected with miR-26a mimic or miR-26a inhibitor. The original images are available in Figure S6. (E) The luciferase activity was detected in HEK293T cells co-transfected HMGB1-WT with miR-26a mimic, miR-26a mimic+pcDNA or miR-26a mimic+pcDNA-TUG1. (F) The expression of HMGB1 protein in NPCs transfected with pcDNA, pcDNA-TUG1, pcDNA-TUG1+miR-NC and pcDNA-TUG1+miR-26a mimic. The original images are available in Figure S7. (G) The expression of HMGB1 protein in NPCs transfected with si-NC, si-TUG1, si-TUG1+inhibitor-NC, si-TUG1+miR-26a inhibitor. The original images are available in Figure S8. *P<0.05, **P<0.01.