Table 1.
Primers and probes used in this study.
| Target gene | Oligonucleotides | Sequence (5′➔3′) | Reference |
|---|---|---|---|
| RdRP | RdRp_SARSr-F | GTGARATGGTCATGTGTGGCGG | (Chan et al., 2020) |
| RdRp_SARSr-P2 | FAM-CAGGTGGAACCTCATCAGGAGATGC-BBQ | ||
| RdRP_SARSr-P1 | FAM-CCAGGTGGWACRTCATCMGGTGATGC-BBQ | ||
| RdRp_SARSr-R | CARATGTTAAASACACTATTAGCATA | ||
| E | E_Sarbeco_F | ACAGGTACGTTAATAGTTAATAGCGT | (Chan et al., 2020) |
| E_Sarbeco_P1 | FAM-ACACTAGCCATCCTTACTGCGCTTCG-BBQ | ||
| E_Sarbeco_R | ATATTGCAGCAGTACGCACACA | ||
| N | N_Sarbeco_F | CACATTGGCACCCGCAATC | (Chan et al., 2020) |
| N_Sarbeco_P | FAM-ACTTCCTCAAGGAACAACATTGCCA-BBQ | ||
| N_Sarbeco_R | GAGGAACGAGAAGAGGCTTG | ||
| RNase P | RNaseP-F | AGATTTGGACCTGCGAGCG | (Emery et al., 2004) |
| RNaseP-R | GAGCGGCTGTCTCCACAAGT | ||
| RNaseP prob | Fam-TTCTGACCTGAAGGCTCTGCGCG-BHQ1 |
W: A/T; R: G/A; M: A/C; S: G/C. FAM: 6-carboxyfluorescein; BBQ: blackberry quencher.