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. Author manuscript; available in PMC: 2020 Oct 7.
Published in final edited form as: J Invest Dermatol. 2016 Dec 23;137(5):1144–1154. doi: 10.1016/j.jid.2016.11.036

Figure 1. GCs promote nuclearization of β-catenin through PKC.

Figure 1.

Primary human keratinocytes control (a) or stimulated with either 30 mM LiCl (b), 1 μM Dex (c) or 100 nM Dex-BSA (f) for 24 hours in presence of 1 μM Ru486 (d and g) or 0.2 μM CC (e and h) are shown. Presence of phospho-β-catenin (Y142) was visualized by immunofluorescence (green; left panels). Nuclei were visualized by staining with PI (red; middle panels) and relative nuclearization was assessed by merging the two images using ImageJ (with co-localization appearing yellow; right panels). CC, calphostin C; Dex, dexamethasone; Dex-BSA, BSA-conjugated dexamethasone; GC, glucocorticoid; LiCl, lithium chloride; PI, propidium iodide; PKC, protein kinase C. Scale bar = 50 μm.