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. Author manuscript; available in PMC: 2020 Oct 7.
Published in final edited form as: J Invest Dermatol. 2016 Dec 23;137(5):1144–1154. doi: 10.1016/j.jid.2016.11.036

Figure 3. mbGR mediated activation of PLCγ/PKC/GSK-3β is present not only in cells of epithelial origin (eye and lung), but also in cells of mesenchymal origin (foot fibroblasts).

Figure 3.

Presence of mbGR mediated activation of PLCy/PKC/GSK-3B signaling cascade was assessed in (a) D407-human retinal epithelial cells and (b) primary human bronchial epithelial cells, as well as in cells of nonepithelial origin like (c) primary human foot fibroblasts. Cells were stimulated with vehicle (DMSO) or 100 nM Dex-BSA (DB) for 30 minutes, and phosphorylation of PLCγ, GR, PKC pan, and GSK-3β were assessed by western blot, with Arpc2 serving as loading control. All quantifications were performed using ImageJ with error bars corresponding to standard deviation from n = 3. Dex-BSA, BSA-conjugated dexamethasone (DB); GR, glucocorticoid receptor; GSK-3β, glycogen synthase kinase 3 beta; mbGR, membranous glucocorticoid receptor; PKC, protein kinase C; PLC, phospholipase C. *P ≤ 0.05 (Student t test).