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. Author manuscript; available in PMC: 2020 Oct 7.
Published in final edited form as: J Invest Dermatol. 2016 Dec 23;137(5):1144–1154. doi: 10.1016/j.jid.2016.11.036

Figure 4. Selective activation of the mbGR results in inhibition of keratinocyte migration.

Figure 4.

Primary human keratinocytes were pretreated with 4 μg/ml mitomycin-C and stimulated with vehicle (DMSO), 25 ng/ml EGF (positive control), 1 μM Dex or 100 nM Dex-BSA (DB). Cells were wounded by a scratch and their migration was assessed at the time of the scratch (0 h) every 2 hours for 48 hours. Representative images at 0, 24, and 48 hours after the initial scratch were used to quantify migration by using Cell Migration Analysis software module (Essen Bioscience) comparing relative wound density, with light gray corresponding to initial wound scratch and dark gray corresponding to repopulation of the wound over time. Error bars correspond to standard deviation from n = 16. *P ≤ 0.05 (Student t test). Dex, dexamethasone; Dex-BSA, BSA-conjugated dexamethasone (DB); mbGR, membranous glucocorticoid receptor.