3.

Effects of bisphenol A (BPA) on adhesion molecule expression.

HTR-8/SVneo cells were treated with BPA (10^–9–10^–6 mol/L) or an equivalent volume of dimethyl sulfoxide (DMSO) (control group) for 48 hours. A: Western blotting was used to detect integrin α5, integrin β1, CD97, vimentin, N-cadherin, E-cadherin, and occludin protein levels. β-Actin was used as the loading control. B and C:Quantitative reverse transcription PCR was used to measure integrin α5, integrin β1, CD97, vimentin, N-cadherin, E-cadherin, and occludin mRNA levels in control group and BPA (10^–7 mol/L) group (n≥3 per group). D: Invasiveness of HTR-8/SVneo cells pre-transfected with integrin β1 and then treated with BPA (10^–7 mol/L) (middle panel), or BPA (10^–7 mol/L) alone (right panel) for 48 hours was determined using transwell assays. The control group (left panel) was treated with DMSO. Scale bar: 50 μm. Data represent means±SEM of at least three independent experiments. ^*P<0.05,^**P<0.01,^***P<0.001, and^****P<0.0001.