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. 2020 Mar 4;18(10):2015–2026. doi: 10.1111/pbi.13360

Figure 2.

Figure 2

Mapping of miR167 target cleavage sites in OsARF12, OsARF17 and OsARF25. (a) Expression pattern of predicted target genes in WT and MIR167a‐OE plants. Ubiquitin 5 was used as a control. Data are presented as means ± SE (n = 3). Significant at **P < 0.01. (b) PCR products of OsARF6, OsARF12, OsARF17 and OsARF25 by RLM‐5'RACE was showed by gel electrophoresis. (c) The sequencing results of 12 clones from the 5′ RACE cleavage products of OsARF6, OsARF12, OsARF17 and OsARF25. The vertical lines represent the nucleotides that base‐pair with miR167a. The arrow points to the OsmiR167‐directed cleavage site at the OsARFs transcript. The numbers above the sequence indicate frequency of 5′RACE clones corresponding to each site.